Molecular basis of control of cell proliferation and the origin of cancer
Morfologia
Agarose
Tumor
N
Ã
O
T
R
A
T
A
D
O
+
C
O
R
T
I
S
O
N
A
C6
P7
ST1
Reversão Fenotípica Tumoral-Normal provocada por tratamento das células de glioma de rato ST1, que são hipersensíveis à cortisona. Estas células ST1 foram derivadas a partir da linhagem C6 de glioma de rato. Por outro lado, as células P7, também derivadas a partir da linhagem C6, são resistentes à cortisona, mantendo o caráter tumoral mesmo na presença deste hormônio.
Refs.
– Armelin & Armelin, 1983 J. Cell Biol. vol. 97, p459-465
– Armelin et al, 1983 J. Cell Biol. 97(2) 455-458 DOI: 10.1083/jcb.97.2.455
Objectives
Identify genes and proteins differentially expressed in tumor cell lines and tumor tissues compared to their respectives normal cell lines and normal tissues. Identify, isolate and characterize tumor stem cells (CSCs) in breast tumors and strains, to understand the origin of these tumors and their resistance to chemotherapeutic drugs. The overall goal is to find new molecular targets for diagnosis and cellular and molecular therapy of these tumors.
Projects in development
- Genomic analysis (biomarkers, DNA microarrays, proteomic analysis by mass spectrometry, fosfoproteomics and regulatory networks);
- Functional genomics (transgenesis, gene edition/inactivation);
- Normal and tumoral cell lines;
- Tumor biomarkers (glioblastoma and breast cancer).
Application areas
- Technological product development for human health;
- College Education;
- Products and biotechnologial processes related to human or animal health.